6W87

K2P2.1 (TREK-1):ML335 complex, 50 mM K+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03320
Spacegroup name	P 21 21 21
Unit cell lengths	66.944, 119.480, 128.393
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.950 - 3.200
R-factor	0.2485
Rwork	0.246
R-free	0.29600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cq6
RMSD bond length	0.010
RMSD bond angle	1.120
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3 (6-FEB-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.330	3.420
High resolution limit [Å]	3.200	3.200
Rmerge	0.107
Number of reflections	17656	3114
<I/σ(I)>	10.3
Completeness [%]	99.9
Redundancy	7.2
CC(1/2)		0.199

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	22-25% PEG400, 100mM HEPES pH=8.0, 1mM CdCl2, 200mM KCl