6W7Y
Crystal structure of SARS-CoV and SARS-CoV-2 reactive human antibody CR3022
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-25 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.065, 201.032, 56.971 |
Unit cell angles | 90.00, 109.44, 90.00 |
Refinement procedure
Resolution | 53.720 - 3.300 |
R-factor | 0.2528 |
Rwork | 0.252 |
R-free | 0.27830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xs7 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.720 | 3.420 |
High resolution limit [Å] | 3.300 | 3.300 |
Rpim | 0.063 | 0.198 |
Number of reflections | 16019 | 2430 |
<I/σ(I)> | 10.2 | 1.6 |
Completeness [%] | 96.5 | |
Redundancy | 1.9 | |
CC(1/2) | 0.991 | 0.935 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M imidazole, pH 6.5, 40% 2-propanol, 15% PEG8000 |