5XS7

Structure of Coxsackievirus A6 (CVA6) virus A-particle in complex with the neutralizing antibody fragment 1D5

Summary for 5XS7

• Entry DOI: 10.2210/pdb5xs7/pdb
• EMDB information: 6757
• Descriptor: Light chain of Fab 1D5, Heavy chain of Fab 1D5, Genome polyprotein, ... (5 entities in total)
• Functional Keywords: coxsackievirus a6, immune-complex, icosahedral, virus
• Biological source: Mus musculus; More
• Cellular location: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Virion : A0A0K2BNC7 A0A0K2BNC7 A0A0K2BNC7
• Total number of polymer chains: 5
• Total formula weight: 112975.76

Authors

Zheng, Q.B.,He, M.Z.,Xu, L.F.,Yu, H.,Li, S.W.,Cheng, T. (deposition date: 2017-06-12, release date: 2017-09-27, Last modification date: 2024-10-30)

Primary citation

Xu, L.,Zheng, Q.,Li, S.,He, M.,Wu, Y.,Li, Y.,Zhu, R.,Yu, H.,Hong, Q.,Jiang, J.,Li, Z.,Li, S.,Zhao, H.,Yang, L.,Hou, W.,Wang, W.,Ye, X.,Zhang, J.,Baker, T.S.,Cheng, T.,Zhou, Z.H.,Yan, X.,Xia, N.
Atomic structures of Coxsackievirus A6 and its complex with a neutralizing antibody
Nat Commun, 8:505-505, 2017

PubMed Abstract: Coxsackievirus A6 (CVA6) has recently emerged as a major cause of hand, foot and mouth disease in children worldwide but no vaccine is available against CVA6 infections. Here, we demonstrate the isolation of two forms of stable CVA6 particles-procapsid and A-particle-with excellent biochemical stability and natural antigenicity to serve as vaccine candidates. Despite the presence (in A-particle) or absence (in procapsid) of capsid-RNA interactions, the two CVA6 particles have essentially identical atomic capsid structures resembling the uncoating intermediates of other enteroviruses. Our near-atomic resolution structure of CVA6 A-particle complexed with a neutralizing antibody maps an immune-dominant neutralizing epitope to the surface loops of VP1. The structure-guided cell-based inhibition studies further demonstrate that these loops could serve as excellent targets for designing anti-CVA6 vaccines.Coxsackievirus A6 (CVA6) causes hand, foot and mouth disease in children. Here the authors present the CVA6 procapsid and A-particle cryo-EM structures and identify an immune-dominant neutralizing epitope, which can be exploited for vaccine development.

PubMed: 28894095
DOI: 10.1038/s41467-017-00477-9

Experimental method

ELECTRON MICROSCOPY (3.8 Å)