6W73
Fab Structure of CD4 Binding Site (CD4bs) Huamn Monoclonal Antibody HmAb64
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-30 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 91.790, 59.352, 75.571 |
Unit cell angles | 90.00, 96.22, 90.00 |
Refinement procedure
Resolution | 45.630 - 2.800 |
R-factor | 0.1822 |
Rwork | 0.178 |
R-free | 0.26270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dee |
RMSD bond length | 0.008 |
RMSD bond angle | 0.974 |
Data reduction software | XDS |
Data scaling software | XDS (1.16_3549) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.630 | 2.970 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmeas | 0.530 | |
Number of reflections | 21339 | 1621 |
<I/σ(I)> | 11.6 | 2.84 |
Completeness [%] | 99.8 | |
Redundancy | 4.2 | |
CC(1/2) | 0.995 | 0.882 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296 | 30% PEG1500, 8%MPD, 0.1M Tris pH8.5 |