6W6F
Structural and catalytic roles of human 18S rRNA methyltransferases DIMT1 in ribosome assembly and translation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X17B1 |
| Synchrotron site | NSLS |
| Beamline | X17B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-15 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.920 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.204, 96.504, 150.019 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.350 - 3.200 |
| R-factor | 0.1759 |
| Rwork | 0.173 |
| R-free | 0.22920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6w6c |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.070 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 14517 | 732 |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 96.7 | |
| Redundancy | 13.2 | |
| CC(1/2) | 0.986 | 0.934 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 285 | 0.2 M Ammonium sulfate, 0.1 M Tris pH 48.5, 20% PEG3350 |
