6W6C

Structural and catalytic roles of human 18S rRNA methyltransferases DIMT1 in ribosome assembly and translation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X17B1
Synchrotron site	NSLS
Beamline	X17B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-15
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.920
Spacegroup name	P 21 21 21
Unit cell lengths	60.076, 95.397, 150.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.180 - 2.380
R-factor	0.1856
Rwork	0.182
R-free	0.24310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zq9
RMSD bond length	0.009
RMSD bond angle	0.959
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.420
High resolution limit [Å]	2.380	2.380
Number of reflections	35302	1716
<I/σ(I)>	16
Completeness [%]	99.9
Redundancy	13
CC(1/2)	0.991	0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		285	0.2 M Ammonium sulfate, 0.1 M Tris pH 48.5, 20% PEG3350