6W4I
APE1 Y269A product complex with abasic DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-22 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.381, 150.233, 156.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.580 - 2.200 |
R-factor | 0.1867 |
Rwork | 0.185 |
R-free | 0.22680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dff |
RMSD bond length | 0.008 |
RMSD bond angle | 1.028 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.580 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmeas | 0.073 | 0.440 |
Number of reflections | 91210 | 2649 |
<I/σ(I)> | 20.6 | |
Completeness [%] | 99.5 | 96.9 |
Redundancy | 5.9 | 3.5 |
CC(1/2) | 0.792 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 24% PEG 3350, 0.1 M Bis Tris Propane (pH 6.5), 0.2 M Sodium Fluoride |