6W3U
APE1 exonuclease substrate complex R237C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2018-06-29 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.177, 65.622, 91.717 |
Unit cell angles | 90.00, 110.52, 90.00 |
Refinement procedure
Resolution | 46.760 - 2.400 |
R-factor | 0.188 |
Rwork | 0.184 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5wn4 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.108 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (1.17.1_3660) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.760 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmeas | 0.089 | 0.971 |
Number of reflections | 28973 | 2048 |
<I/σ(I)> | 12 | |
Completeness [%] | 92.9 | |
Redundancy | 3.5 | |
CC(1/2) | 0.683 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 7% PEG20000, 100 mM sodium citrate, 15% glycerol, 5 mM calcium chloride |