6W2M
Abortive ternary complex crystal structure of DNA polymerase Beta with 8OG-dC base pair at the primer terminus and flipped out dA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-06 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.751, 80.462, 55.612 |
| Unit cell angles | 90.00, 107.75, 90.00 |
Refinement procedure
| Resolution | 23.927 - 1.998 |
| R-factor | 0.1962 |
| Rwork | 0.193 |
| R-free | 0.23810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rjh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.936 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 1.998 | 1.998 |
| Rmerge | 0.042 | 0.341 |
| Number of reflections | 28097 | 2766 |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 97.3 | 97 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 16-18% PEG 3350 |
