6W1I
Re-interpretation of ppGpp (G4P) electron density in the deposited crystal structure of Xanthine phosphoribosyltransferase (XPRT) (1Y0B).
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 198 |
| Detector technology | CCD |
| Collection date | 2003-02-18 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.503, 115.902, 73.985 |
| Unit cell angles | 90.00, 94.30, 90.00 |
Refinement procedure
| Resolution | 34.230 - 1.800 |
| R-factor | 0.1621 |
| Rwork | 0.160 |
| R-free | 0.20830 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.896 |
| Data reduction software | d*TREK |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.230 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.100 | |
| Number of reflections | 58401 | 600000 |
| <I/σ(I)> | 0 | 0.1 |
| Completeness [%] | 88.5 | 49.7 |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 100 | BIS-TRIS, NaCl, PEG3350, PH 5.5 |
