6W0Q
APE1 endonuclease product complex D148E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-31 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.435, 60.496, 72.982 |
| Unit cell angles | 83.51, 79.62, 88.61 |
Refinement procedure
| Resolution | 24.810 - 1.890 |
| R-factor | 0.1872 |
| Rwork | 0.186 |
| R-free | 0.22290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dff |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.025 |
| Data reduction software | PHENIX (1.17.1_3660) |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.810 | 1.930 |
| High resolution limit [Å] | 1.890 | 1.900 |
| Rmeas | 0.087 | 0.545 |
| Number of reflections | 111259 | 5232 |
| <I/σ(I)> | 18 | 2.3 |
| Completeness [%] | 99.8 | |
| Redundancy | 4.1 | |
| CC(1/2) | 0.773 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 10% PEG20000, 100 mM sodium citrate, pH 5.0, and 200 mM MgCl2 |
