6VZY

Crystal structure of SznF from Streptomyces achromogenes var. streptozoticus NRRL 2697 with a diiron(II) central domain cofactor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.03319
Spacegroup name	P 21 21 21
Unit cell lengths	57.953, 105.848, 150.591
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.420 - 1.660
R-factor	0.1911
Rwork	0.190
R-free	0.22180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m9r
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.690
High resolution limit [Å]	1.660	1.660
Number of reflections	109965	109902
<I/σ(I)>	23.6
Completeness [%]	96.8
Redundancy	5.8
CC(1/2)	0.996	0.677

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	16% PEG4000, 0.1 M magnesium chloride, 0.1 M Tris, pH 8.5