6VZU

TTLL6 bound to alpha-elongation analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	75.263, 110.631, 173.370
Unit cell angles	90.00, 90.24, 90.00

Refinement procedure

Resolution	46.630 - 1.980
R-factor	0.175
Rwork	0.174
R-free	0.19300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ylr
RMSD bond length	0.008
RMSD bond angle	0.946
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.630	2.010
High resolution limit [Å]	1.980	1.980
Rmerge	0.103	0.495
Rmeas	0.116	0.577
Rpim	0.045	0.239
Number of reflections	193528	18563
<I/σ(I)>	16.23	2.12
Completeness [%]	97.9	94.57
Redundancy	6.4	5.4
CC(1/2)	0.990	0.845

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	290	100 mM Sodium Citrate, pH 6.2, 200 mM MgCl2, 8-12% Peg 20000