6VYO
Crystal structure of RNA binding domain of nucleocapsid phosphoprotein from SARS coronavirus 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-25 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.238, 77.430, 114.039 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.050 - 1.700 |
R-factor | 0.1615 |
Rwork | 0.159 |
R-free | 0.20530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2OFZ.pdb |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
Rmerge | 0.131 | 0.070 | 1.134 |
Rmeas | 0.137 | 0.073 | 1.192 |
Rpim | 0.038 | 0.020 | 0.357 |
Total number of observations | 798795 | ||
Number of reflections | 64592 | 3480 | 3101 |
<I/σ(I)> | 6.9 | ||
Completeness [%] | 99.7 | 99.6 | 97.3 |
Redundancy | 12.4 | 12.6 | 10.2 |
CC(1/2) | 0.997 | 0.865 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | 20.0% PEG6000, 0.1M MES, 10.0 mM Zinc chloride |