6VYJ
Human UHRF1 TTD domain in complex with a fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 83 |
Detector technology | PIXEL |
Collection date | 2018-02-06 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97741 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.280, 55.350, 66.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.480 - 1.390 |
R-factor | 0.199 |
Rwork | 0.198 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qqd |
RMSD bond length | 0.008 |
RMSD bond angle | 0.984 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.260 | 1.410 |
High resolution limit [Å] | 1.390 | 1.390 |
Number of reflections | 30553 | 1464 |
<I/σ(I)> | 7 | |
Completeness [%] | 100.0 | |
Redundancy | 6 | |
CC(1/2) | 0.993 | 0.364 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 50 mM Bis-Tris, pH 5.75, 35 % w/v PEG 3350. The crystals were cryo-preserved by 25% glucose |