6VVV
Crystal structure of a Mycobacterium smegmatis transcription initiation complex with Rifampicin-resistant RNA polymerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-12-11 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 129.748, 162.344, 137.155 |
Unit cell angles | 90.00, 111.27, 90.00 |
Refinement procedure
Resolution | 49.830 - 3.200 |
R-factor | 0.2348 |
Rwork | 0.234 |
R-free | 0.26190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5tw1 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (v0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 60.000 | 60.000 | 3.310 |
High resolution limit [Å] | 3.200 | 6.890 | 3.200 |
Rmerge | 0.147 | 0.036 | 2.818 |
Rmeas | 0.167 | 0.040 | 3.320 |
Rpim | 0.077 | 0.018 | 1.711 |
Total number of observations | 372558 | ||
Number of reflections | 85082 | 8654 | 7994 |
<I/σ(I)> | 4.7 | ||
Completeness [%] | 98.3 | 98.3 | 93 |
Redundancy | 4.4 | 4.5 | 3.2 |
CC(1/2) | 0.999 | 0.185 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 0.1 M Bis-Tris, pH 6.0, 0.2 M lithium sulfate, 20% w/v PEG3350, 2.5% v/v ethylene glycol, 1% v/v DMSO |