6VTW
De novo protein design enables the precise induction of RSV-neutralizing antibodies
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 148.224, 148.224, 45.046 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.060 - 2.600 |
R-factor | 0.2147 |
Rwork | 0.207 |
R-free | 0.28270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3o41 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.926 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.060 | 2.693 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.073 | 0.854 |
Rmeas | 0.079 | 0.963 |
Rpim | 0.030 | 0.433 |
Number of reflections | 17472 | 1567 |
<I/σ(I)> | 17.14 | 1.75 |
Completeness [%] | 91.0 | 89.58 |
Redundancy | 6.5 | 4.7 |
CC(1/2) | 0.987 | 0.116 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | containing 0.2 M Magnesium acetate, 0.1 M Sodium cacodylate pH 6.5, 20 %(w/v) PEG 8000 |