6VTP

Crystal structure of G16C human Galectin-7 mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-19
Detector	RAYONIX MX300HE
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	53.600, 67.150, 71.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.080 - 2.300
R-factor	0.2189
Rwork	0.214
R-free	0.26060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bkz
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.7.12)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.080	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.151	2.416
Rmeas	0.159	2.545
Total number of observations	115145
Number of reflections	11900	1154
<I/σ(I)>	14.5	1.2
Completeness [%]	99.0	98.13
Redundancy	9.68	10.01
CC(1/2)	0.999	0.569

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295.15	0.1 M Sodium chloride, 0.1M Tris pH 8, 20 % PEG 3350, 15 % Glycerol