6VTM
Human Cathepsin-G Inhibited by S. aureus EapH1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97243 |
Spacegroup name | P 1 |
Unit cell lengths | 59.229, 72.902, 88.906 |
Unit cell angles | 113.58, 91.17, 106.24 |
Refinement procedure
Resolution | 34.350 - 1.600 |
R-factor | 0.175 |
Rwork | 0.174 |
R-free | 0.20900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1au8 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (V1.14-3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.112 | 0.857 |
Number of reflections | 153604 | |
<I/σ(I)> | 15.8 | |
Completeness [%] | 90.5 | 89.6 |
Redundancy | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M lithium sulfate monohydrate, 0.1 M Tris, pH 8.5, 25% w/v PEG3350 |