6VTM
Human Cathepsin-G Inhibited by S. aureus EapH1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97243 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.229, 72.902, 88.906 |
| Unit cell angles | 113.58, 91.17, 106.24 |
Refinement procedure
| Resolution | 34.350 - 1.600 |
| R-factor | 0.175 |
| Rwork | 0.174 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1au8 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (V1.14-3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.112 | 0.857 |
| Number of reflections | 153604 | |
| <I/σ(I)> | 15.8 | |
| Completeness [%] | 90.5 | 89.6 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M lithium sulfate monohydrate, 0.1 M Tris, pH 8.5, 25% w/v PEG3350 |
