6VSK

Crystal structure of the P-Rex1 DEP1 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-11-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 63 2 2
Unit cell lengths	103.152, 103.152, 67.981
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.620 - 3.120
R-factor	0.1855
Rwork	0.183
R-free	0.23320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o7f
RMSD bond length	0.009
RMSD bond angle	1.796
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.170
High resolution limit [Å]	3.120	3.120
Rmeas	0.152	0.602
Rpim	0.049	0.316
Number of reflections	4105	185
<I/σ(I)>	19	1.3
Completeness [%]	99.3	95.4
Redundancy	8.4	3
CC(1/2)		0.713

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	0.1 M HEPES, pH 6.5, 45% w/v poly(acrylic acid sodium salt)2100