6VRG

Structure of HIV-1 integrase with native amino-terminal sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92013
Spacegroup name	P 43 21 2
Unit cell lengths	102.919, 102.919, 279.203
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	96.570 - 2.400
R-factor	0.22525453281
Rwork	0.225
R-free	0.25285
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k6y
RMSD bond length	0.004
RMSD bond angle	0.589
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	96.650	2.490
High resolution limit [Å]	2.400	2.400
Rpim	0.110	0.318
Number of reflections	59665	5823
<I/σ(I)>	8.1	1.6
Completeness [%]	100.0	100
Redundancy	26.6	26.6
CC(1/2)	0.990	0.289

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	0.7 M NaH2PO4/1.0 M K2HPO4, 0.1M Na acetate