6VR2

Aminoglycoside N-2'-Acetyltransferase-Ia [AAC(2')-Ia] in complex with acetylated-tobramycin and CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	LIQUID ANODE
Source details	BRUKER METALJET
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-02
Detector	Bruker PHOTON II
Wavelength(s)	1.34
Spacegroup name	P 32 2 1
Unit cell lengths	72.798, 72.798, 146.321
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.470 - 1.770
R-factor	0.1682
Rwork	0.167
R-free	0.19240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jzs
RMSD bond length	0.006
RMSD bond angle	0.905
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	PHENIX ((1.17_3644: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.470	1.833
High resolution limit [Å]	1.770	1.770
Number of reflections	44518	4369
<I/σ(I)>	22.65	2.52
Completeness [%]	99.9
Redundancy	2
CC(1/2)	0.999	0.654

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.4	277.15	MPD, sodium acetate, tobramycin, Acetyl CoA