6VPQ
Crystal structure of the C-terminal domain of DENR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-08 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.670, 47.260, 95.500 |
Unit cell angles | 90.00, 106.29, 90.00 |
Refinement procedure
Resolution | 91.667 - 1.740 |
R-factor | 0.1812 |
Rwork | 0.178 |
R-free | 0.24730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vyc |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 91.667 | 91.667 | 1.790 |
High resolution limit [Å] | 1.740 | 7.780 | 1.740 |
Rmerge | 0.105 | 0.030 | 6.596 |
Rmeas | 0.121 | 0.035 | 7.505 |
Total number of observations | 249082 | ||
Number of reflections | 56630 | 665 | 4138 |
<I/σ(I)> | 7.84 | 35.88 | 0.18 |
Completeness [%] | 98.9 | 94.5 | 99.4 |
Redundancy | 4.398 | 4.018 | 4.363 |
CC(1/2) | 0.997 | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 285 | 0.1 M CaCl2, 15% PEG 400, 0.1 M Sodium acetate, pH 5.0 |