6VPH

TPX2 residues 7-20 fused to Aurora A residues 116-389 modified with cacodylate and in complex with AMP-PNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-09-09
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 61 2 2
Unit cell lengths	89.081, 89.081, 183.806
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.540 - 2.140
R-factor	0.1894
Rwork	0.187
R-free	0.21650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ol5
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.220
High resolution limit [Å]	2.140	4.611	2.140
Rmerge	0.062	0.040	0.286
Rmeas	0.065	0.042	0.335
Rpim	0.019	0.011	0.170
Total number of observations	233292
Number of reflections	24359	2714	2139
<I/σ(I)>	49.5
Completeness [%]	98.8	99.8	89.1
Redundancy	9.6	13.5	3.5
CC(1/2)		0.999	0.891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	298	0.1 M sodium cacodylate pH 6, 2 M NaCl, 9% PEG 3350