6VNK
JAK2 JH1 in complex with PN4-073
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 111.672, 70.434, 112.786 |
Unit cell angles | 90.00, 90.61, 90.00 |
Refinement procedure
Resolution | 40.907 - 2.000 |
R-factor | 0.2186 |
Rwork | 0.218 |
R-free | 0.24420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xa4 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14-3260_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.907 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmeas | 0.115 | |
Number of reflections | 199608 | 11191 |
<I/σ(I)> | 9.9 | |
Completeness [%] | 93.3 | 94.8 |
Redundancy | 2.8 | |
CC(1/2) | 1.000 | 0.410 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.1 M Bis-Tris pH 5.5, 0.2 M NaCl, 25% PEG 3350 |