6VME
Human ESCRT-I heterotetramer headpiece
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-01 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.11583 |
Spacegroup name | P 61 |
Unit cell lengths | 162.584, 162.584, 139.715 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 99.180 - 2.190 |
R-factor | 0.1943 |
Rwork | 0.193 |
R-free | 0.23960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2f66 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.885 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.180 | 2.269 |
High resolution limit [Å] | 2.190 | 2.190 |
Rpim | 0.042 | |
Number of reflections | 107460 | 10167 |
<I/σ(I)> | 17.22 | |
Completeness [%] | 99.5 | 95.09 |
Redundancy | 20 | |
CC(1/2) | 0.999 | 0.281 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291.15 | 2 % w/v gamma-Polyglutamic acid (gamma-PGA), 100 mM sodium formate, 100 mM sodium acetate pH 5.0 |