6VLK
A varicella-zoster virus glycoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 118.318, 118.318, 749.026 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.222 - 2.453 |
| R-factor | 0.1832 |
| Rwork | 0.181 |
| R-free | 0.22840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gum |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.123 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.520 | 2.590 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.073 | 0.516 |
| Number of reflections | 74955 | 10367 |
| <I/σ(I)> | 19.8 | 3.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.8 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Na acetate pH 4.6, 20% PEG 400, 6% ethylene glycol |
