6VLD
Crystal structure of human alpha 1,6-fucosyltransferase, FUT8 bound to GDP and A2SGP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9536 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 208.307, 68.453, 249.983 |
Unit cell angles | 90.00, 111.21, 90.00 |
Refinement procedure
Resolution | 48.550 - 2.280 |
R-factor | 0.193 |
Rwork | 0.191 |
R-free | 0.22390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2de0 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.650 | 2.320 |
High resolution limit [Å] | 2.280 | 2.280 |
Rmerge | 0.103 | 1.517 |
Rmeas | 0.114 | 1.676 |
Rpim | 0.047 | 0.700 |
Total number of observations | 849036 | 41177 |
Number of reflections | 150224 | 7302 |
<I/σ(I)> | 10.2 | 1.1 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 5.7 | 5.6 |
CC(1/2) | 0.998 | 0.435 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 281 | 2 M NH4SO4, 0.2 M NaCl, 0.1 M sodium cacodylate, pH 6.5 |