6VKM

Crystal Structure of Stabilized GP from Makona Variant of Ebola Virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-10-26
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97918
Spacegroup name	H 3 2
Unit cell lengths	113.110, 113.110, 393.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.530 - 3.500
R-factor	0.2849
Rwork	0.284
R-free	0.30310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jq3
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.5.7)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.530	47.530	3.830
High resolution limit [Å]	3.500	8.570	3.500
Rmerge	0.346	0.156	0.743
Rmeas	0.391	0.174	0.838
Rpim	0.177	0.076	0.378
Total number of observations	45250	3273	10985
Number of reflections	10491	727	2527
<I/σ(I)>	3.4	6	2
Completeness [%]	84.7	80.6	86.7
Redundancy	4.3	4.5	4.3
CC(1/2)	0.913	0.972	0.564

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	293	9.8% PEG 6000, 0.1 M Sodium citrate pH 5.2, 3% glycerol