6VI2
Structure of the unaligned Fab4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 292 |
Detector technology | PIXEL |
Collection date | 2019-12-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.765, 65.843, 107.419 |
Unit cell angles | 90.00, 99.83, 90.00 |
Refinement procedure
Resolution | 14.990 - 1.150 |
R-factor | 0.1576 |
Rwork | 0.158 |
R-free | 0.17090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5eu7 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.190 |
High resolution limit [Å] | 1.150 | 1.150 |
Rpim | 0.027 | 0.559 |
Number of reflections | 293620 | 18649 |
<I/σ(I)> | 50.4 | 1.9 |
Completeness [%] | 93.8 | 59.8 |
Redundancy | 4.7 | 3 |
CC(1/2) | 0.511 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 0.1 M Tris pH 8.5, 25% (w/v) Polyethylene glycol 3350 |