6VGR
Crystal Structure of Human Dipeptidase 3 in Complex with Fab of SC-003
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 297 |
Detector technology | PIXEL |
Collection date | 2018-10-31 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 63 |
Unit cell lengths | 167.030, 167.030, 116.380 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.120 - 2.840 |
R-factor | 0.193 |
Rwork | 0.190 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6vgo |
RMSD bond length | 0.010 |
RMSD bond angle | 1.120 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.120 | 2.890 |
High resolution limit [Å] | 2.840 | 2.840 |
Rmeas | 0.091 | |
Rpim | 0.356 | |
Number of reflections | 42049 | 2173 |
<I/σ(I)> | 20 | |
Completeness [%] | 96.2 | |
Redundancy | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 297 | 0.1 M calcium acetate, 0.1 M sodium acetate pH 4.5, 10% w/v PEG4000 |