6VGE
Crystal structure of the DNA binding domains of human transcription factor ERG, human Runx2 bound to core binding factor beta (Cbfb), in complex with 16mer DNA CAGAGGATGTGGCTTC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-22 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 103.917, 103.917, 322.912 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.000 - 4.250 |
| R-factor | 0.3128 |
| Rwork | 0.313 |
| R-free | 0.31720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6vgd |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.177 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.320 |
| High resolution limit [Å] | 4.250 | 4.250 |
| Rmerge | 0.103 | 1.382 |
| Number of reflections | 7550 | 367 |
| <I/σ(I)> | 17.9 | |
| Completeness [%] | 94.7 | |
| Redundancy | 6.1 | |
| CC(1/2) | 0.785 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.6 M K/Na Tartrate, 0.1 M Hepes, pH7.0 |
