6VGD
Crystal structure of the DNA binding domain (DBD) of human FLI1 and the complex of the DBD of human Runx2 with core binding factor beta (Cbfb), in complex with 16mer DNA CAGAGGATGTGGCTTC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-22 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 104.513, 104.513, 322.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.000 - 4.200 |
| R-factor | 0.2947 |
| Rwork | 0.295 |
| R-free | 0.29320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6vg8 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.257 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.130 | 0.954 |
| Number of reflections | 17612 | 825 |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 99.3 | |
| Redundancy | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.6 M K/Na Tartrate, 0.1 M Hepes, pH7.0 |
