6VG8
Crystal structure of the DNA binding domains of human FLI1 and Runx2 in complex with 16-mer DNA CAGAGGATGTGGCTTC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-10 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.000 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 104.427, 104.427, 326.382 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.210 - 4.310 |
R-factor | 0.2736 |
Rwork | 0.273 |
R-free | 0.28270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jvt |
RMSD bond length | 0.003 |
RMSD bond angle | 1.221 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.310 |
High resolution limit [Å] | 3.250 | 3.250 |
Rmerge | 0.131 | 0.433 |
Number of reflections | 16635 | 696 |
<I/σ(I)> | 12.9 | |
Completeness [%] | 93.8 | |
Redundancy | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 7% w/v PEG 4000, 50 mM Na Citrate, pH 5.6, 4% v/v isopropanol |