6VG2

Crystal structure of the DNA binding domain of human transcription factor FLI1 in complex with 16-mer DNA CAGAGGATGTGGCTTC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-19
Detector	RAYONIX MX300-HS
Wavelength(s)	1.000
Spacegroup name	I 21 21 21
Unit cell lengths	79.422, 90.732, 165.204
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 3.900
R-factor	0.2495
Rwork	0.247
R-free	0.29430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jvt
RMSD bond length	0.002
RMSD bond angle	1.063
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.710
High resolution limit [Å]	3.650	3.650
Rmerge	0.122
Number of reflections	6615	287
<I/σ(I)>	8.1
Completeness [%]	95.8
Redundancy	5.2
CC(1/2)		0.493

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	1.6 M ammonium sulfate