6VFW
Crystal structure of human delta protocadherin 10 EC1-EC4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-29 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 346.550, 73.193, 246.328 |
Unit cell angles | 90.00, 132.06, 90.00 |
Refinement procedure
Resolution | 19.990 - 3.600 |
R-factor | 0.2773 |
Rwork | 0.277 |
R-free | 0.29480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6vfq |
RMSD bond length | 0.012 |
RMSD bond angle | 0.783 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.710 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmerge | 0.123 | 4.649 |
Rmeas | 0.146 | 5.476 |
Number of reflections | 52814 | 4543 |
<I/σ(I)> | 5.4 | 0.4 |
Completeness [%] | 98.2 | 98.3 |
Redundancy | 3.4 | 3.6 |
CC(1/2) | 1.000 | 0.290 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296 | 12% (w/v) PEG 8000, 20% (v/v) ethylene glycol, 0.1M Tris-bicine buffer pH8.5, 10% 0.02 M D-glucose, 0.02 M D-mannose, 0.02 M D-galactose, 0.02 M L-fucose, 0.02 M D-xylose, 0.02 M N-acetyl-D-glucosamine with no further cryoprotection |