6VFP

Crystal structure of human protocadherin 1 EC1-EC4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-25
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 61 2 2
Unit cell lengths	148.595, 148.595, 149.846
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.870 - 3.200
R-factor	0.224
Rwork	0.222
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dzq 4zpl
RMSD bond length	0.008
RMSD bond angle	0.541
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.420
High resolution limit [Å]	3.200	3.200
Rmerge	0.120	1.685
Rmeas	0.120	1.730
Rpim	0.028	0.394
Number of reflections	16684	2938
<I/σ(I)>	15.9	2.1
Completeness [%]	100.0	100
Redundancy	18.3	19.1
CC(1/2)	1.000	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	296	8.5% (w/v) PEG 4000, 20% (v/v) glycerol, 0.1M tris-bicine buffer pH8.5, 0.02 M D-glucose, 0.02 M D-mannose, 0.02 M D-galactose, 0.02 M L-fucose, 0.02 M D-xylose, 0.02 M N-acetyl-D-glucosamine