6VFA

DNA Polymerase Mu, 8-oxorGTP:Ct Ground State Ternary Complex, 50 mM Mn2+ (15 min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.079, 62.215, 118.925
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.959 - 1.760
R-factor	0.1764
Rwork	0.175
R-free	0.20290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.790
High resolution limit [Å]	1.757	4.780	1.760
Rmerge	0.105	0.071	0.847
Rmeas	0.117	0.080	0.951
Rpim	0.049	0.035	0.423
Number of reflections	44808	2393	2220
<I/σ(I)>	8.2
Completeness [%]	99.1	97.3	99.7
Redundancy	5.4	5.2	4.8
CC(1/2)		0.983	0.588

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000