6VF7

DNA Polymerase Mu, 8-oxodGTP:At Ground State Ternary Complex, 50 mM Mn2+ (15 min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.159, 68.927, 110.446
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.035 - 1.871
R-factor	0.1673
Rwork	0.166
R-free	0.19670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.900
High resolution limit [Å]	1.870	5.070	1.870
Rmerge	0.093	0.046	0.930
Rmeas	0.104	0.051	1.038
Rpim	0.045	0.022	0.454
Total number of observations	194774
Number of reflections	38515	2099	1891
<I/σ(I)>	8.4
Completeness [%]	99.5	99.1	99.8
Redundancy	5.1	5.2	4.8
CC(1/2)		0.997	0.605

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000