6VF4

DNA Polymerase Mu, 8-oxorGTP:At Reaction State Ternary Complex, 50 mM Mn2+ (30 min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.085, 68.757, 110.531
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.475 - 1.750
R-factor	0.1692
Rwork	0.168
R-free	0.19230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.780
High resolution limit [Å]	1.750	4.750	1.750
Rmerge	0.062	0.034	0.970
Rmeas	0.067	0.037	1.066
Rpim	0.026	0.014	0.435
Total number of observations	305822
Number of reflections	46821	2539	2297
<I/σ(I)>	11.3
Completeness [%]	99.7	98.8	99.7
Redundancy	6.5	6.3	5.8
CC(1/2)		0.999	0.723

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000