6VE5
X-ray structure of human REV7 in complex with Shieldin3 (residues 41-74)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-02-15 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 59.990, 59.990, 132.470 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.250 - 2.000 |
R-factor | 0.2178 |
Rwork | 0.215 |
R-free | 0.23860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3abd |
RMSD bond length | 0.004 |
RMSD bond angle | 0.652 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.250 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.083 | 1.193 |
Rmeas | 0.085 | 1.222 |
Rpim | 0.018 | 0.262 |
Number of reflections | 19399 | 1337 |
<I/σ(I)> | 40.89 | 4.11 |
Completeness [%] | 98.4 | |
Redundancy | 22.6 | |
CC(1/2) | 0.999 | 0.847 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 288 | Protein complex was in 5 mM HEPES, pH 7.4, 100 mM NaCl, 5 mM DDT at 25 mg/mL;Reservoir solution was 0.1 M MES monohydrate, pH 6.5, 1.4 M MgSO4.6H20;Cryoprotection was done in 50% PEG 400 |