6VE1
Crystal structure of endo-beta-N-acetylglucosaminidase H at high pH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 93.480, 99.480, 135.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.031 - 2.100 |
R-factor | 0.2225 |
Rwork | 0.221 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1edt |
Data scaling software | Aimless |
Phasing software | BALBES |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.031 | 2.175 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 74108 | 7330 |
<I/σ(I)> | 5.89 | |
Completeness [%] | 99.7 | |
Redundancy | 2 | |
CC(1/2) | 0.986 | 0.618 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 9 | 295 | PEG20000, magnesium nitrate, TAPS, pH 9.0 |