6VDJ
Crystal structure of ancestral apicomplexan lactate dehydrogenase with sulfate and NADH4.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2019-10-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.115866 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 84.695, 84.695, 196.007 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.790 - 2.000 |
| R-factor | 0.2507 |
| Rwork | 0.251 |
| R-free | 0.26190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4plg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.822 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.790 | 1.520 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmeas | 0.160 | 383.600 |
| Number of reflections | 146503 | 105617 |
| <I/σ(I)> | 5.02 | |
| Completeness [%] | 96.4 | 76.2 |
| Redundancy | 8.84 | 2.94 |
| CC(1/2) | 0.990 | 0.009 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20mg/mL protein, 2mM NADH4; well solution: 9% v/v 2-Propanol, 1.4M Ammonium Sulfate |
