6VCZ
Crystal structure of Arabidopsis thaliana S-adenosylmethionine Synthase 2 (AtMAT2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-11-01 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.798, 99.276, 86.930 |
| Unit cell angles | 90.00, 109.74, 90.00 |
Refinement procedure
| Resolution | 42.440 - 1.520 |
| R-factor | 0.1605 |
| Rwork | 0.160 |
| R-free | 0.17990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6vcw |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.003 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.440 | 1.610 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.054 | 0.609 |
| Number of reflections | 154106 | 24808 |
| <I/σ(I)> | 16.26 | 2.15 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 4.12 | 3.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 0.12 M Alcohols (1,6-hexanediol; 1-butanol; 1,2-propanediol; 2-propanol; 1,4-butanediol; 1,3-propanediol), 0.1 M HEPES and MOPS buffer at pH 7.5, 20% mmPEG500, 10% PEG 20000 |
