6VCS

SRA domain of UHRF1 in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-23
Detector	RIGAKU SATURN A200
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	51.995, 113.809, 133.946
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.600 - 1.700
R-factor	0.2378
Rwork	0.237
R-free	0.25750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bi7
RMSD bond length	0.006
RMSD bond angle	0.794
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.600	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.086	1.068
Rmeas	0.093	1.207
Number of reflections	87819	3976
<I/σ(I)>	11.3	1.2
Completeness [%]	99.3
Redundancy	6.8	4.5
CC(1/2)	0.998	0.660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.1M sodium malonate, 12% PEG3350