6VBY
Cinnamate 4-hydroxylase (C4H1) from Sorghum bicolor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2019-09-28 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 132.278, 132.278, 79.431 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 65.275 - 1.700 |
R-factor | 0.1988 |
Rwork | 0.199 |
R-free | 0.21480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4r1z |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.275 | 1.761 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 87984 | 8712 |
<I/σ(I)> | 4.75 | 1.12 |
Completeness [%] | 99.9 | |
Redundancy | 18.6 | |
CC(1/2) | 0.995 | 0.462 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1 M HEPES, pH 7.5, 12% (v/v) isopropanol and 14% (w/v) PEG 2,000 |