6VBR
The crystal structure of the 2009 H1N1/California PA endonuclease wild type in complex with SJ000986248
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 90.790, 90.790, 134.118 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.860 - 2.750 |
| R-factor | 0.2397 |
| Rwork | 0.239 |
| R-free | 0.25480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vpt |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.978 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (dev_3965) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.180 | 2.880 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.060 | 1.173 |
| Rmeas | 0.064 | 1.242 |
| Rpim | 0.022 | 0.404 |
| Number of reflections | 7619 | 983 |
| <I/σ(I)> | 18.6 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.7 | 9.3 |
| CC(1/2) | 0.999 | 0.746 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M HEPES PH 7.8, 1 M AMMONIUM SULFATE, 10 MM MNCL2, 10 MM MGCL2, 0.5% PVP K15, PH 6.5 |
