6VBG

Lactose permease complex with thiodigalactoside and nanobody 9043

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	103
Detector technology	PIXEL
Collection date	2017-09-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.115
Spacegroup name	P 65
Unit cell lengths	151.270, 151.270, 182.480
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.060 - 2.800
R-factor	0.2401
Rwork	0.239
R-free	0.27480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6c9w
RMSD bond length	0.003
RMSD bond angle	0.700
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.100	50.000	2.900
High resolution limit [Å]	2.800	30.000	2.800
Rmerge	0.216	0.012	2.724
Rmeas	0.265	0.016	3.341
Number of reflections	57178	22	5725
<I/σ(I)>	5.8	57.94	0.45
Completeness [%]	98.3	53.7	99.2
Redundancy	2.899	3	2.761
CC(1/2)	0.987	0.999	0.111

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.07 M sodium chloride, 0.05 M sodium citrate pH 4.5,22% v/v PEG 400