6V9T

Tudor domain of TDRD3 in complex with a small molecule

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-28
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	H 3 2
Unit cell lengths	83.855, 83.855, 114.045
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.849 - 2.154
Rwork	0.196
R-free	0.22890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pmt
RMSD bond length	0.017
RMSD bond angle	2.025
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.850	44.850	2.220
High resolution limit [Å]	2.150	8.880	2.150
Rmerge	0.104	0.047	0.982
Rmeas	0.109	0.050	1.030
Number of reflections	8558	141	703
<I/σ(I)>	18.9	53.7	2.6
Completeness [%]	99.8
Redundancy	11.1	9.1	11.1
CC(1/2)	0.998	0.999	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1.2 M sodium citrate, 0.1 M Tris-HCl, pH 8.5