6V8F

Crystal structure of recombinat mutant Q185R of human fumarase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-09
Detector	PSI PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	P 65 2 2
Unit cell lengths	190.489, 190.489, 115.184
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	95.240 - 2.300
R-factor	0.1518
Rwork	0.150
R-free	0.18110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5upp
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.244	2.370
High resolution limit [Å]	2.300	2.300
Number of reflections	54953	4425
<I/σ(I)>	19.6
Completeness [%]	100.0
Redundancy	32.4
CC(1/2)	0.999	0.927

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	294	0.1 M HEPES, 0.2 M Lithium sulfate, 25% (m/v) PEG 3350